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Rekombinant Anti-Human IgG1 Antikörper [RM117] (Biotin) (A121269)

ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.com ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.com ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.com ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121337) - Antibodies.com
Rekombinant Antikörper
$480

Monoklonal [RM117] Kaninchen Antikörper gegen IgG1 (Biotin) für ICC, IHC, Flow Cytometry und ELISA.

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Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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+1 (314) 370-6046
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orders@antibodies.com

Produktname
Anti-Human IgG1 Antibody [RM117] (Biotin)
Beschreibung
Recombinant rabbit monoclonal [RM117] antibody to Human IgG1 (Biotin).
Spezifität
This antibody recognizes the heavy chain of human IgG1.
Anwendungen
ICC, IHC, Flow Cytometry, ELISA
Verdünnungen
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Reaktivität
Human
Kreuzreaktivität
There is no cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen
Synthetic peptide corresponding to the hinge region of human IgG1.
Wirt
Rabbit
Klonalität
Monoclonal
Klon
RM117
Isotyp
IgG
Konjugat

Biotin

Reinigung
Protein A chromatography.
Konzentration
1 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
Ig gamma 1 chain C region, IgG heavy chain locus, IGHG1, Immunoglobulin Gm1, Immunoglobulin heavy constant gamma 1 (G1m marker)
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.comBild Vergrößern
Figure 1: Anti-Human IgG1 Antibody [RM117] (Biotin) (A121269)
Sandwich ELISA using Anti-Human IgG1 Antibody [RM117] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.comBild Vergrößern
Figure 2: Anti-Human IgG1 Antibody [RM117] (Biotin) (A121269)
Sandwich ELISA using Anti-Human IgG1 Antibody [RM117] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121269) - Antibodies.comBild Vergrößern
Figure 3: Anti-Human IgG1 Antibody [RM117] (Biotin) (A121269)
ELISA of human immunoglobulins shows Anti-Human IgG1 Antibody [RM117] only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgG1 Antibody [RM117] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Human IgG1 Antibody (Biotin) [RM117] (A121337) - Antibodies.comBild Vergrößern
Figure 4: Anti-Human IgG1 Antibody [RM117] (Biotin) (A121269)
A titer ELISA using Anti-Human IgG1 Antibody [RM117]. The plate was coated with different amounts of human IgG1. A serial dilution of Anti-Human IgG1 Antibody [RM117] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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