Rekombinant Anti-Human IgG1 Antikörper [RM117] (A121337)

Detection of human IgG1 in monkey serum, using Anti-Human IgG1 Antibody [RM117] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.

Sandwich ELISA using Anti-Human IgG1 Antibody [RM117] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.

ELISA showing Anti-Human IgG1 Antibody [RM117] reacts only to human IgG1, and not to any other IgG subclasses (IgG2, IgG3, or IgG4), and there is no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

A titer ELISA using Anti-Human IgG1 Antibody [RM117]. The plate was coated with different amounts of human IgG1. A serial dilution of Anti-Human IgG1 Antibody [RM117] was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.