Human IGFBP-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IGFBP-3 in the samples. A monoclonal antibody specific for human IGFBP-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IGFBP-3, and IGFBP-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGFBP-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

2.16 pg/ml

Detektionsbereich

125 - 8000 pg/ml

Spezifität

This kit can assay both natural and recombinant human IGFBP-3.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Acid stable subunit of the 140 K IGF complex, Binding protein 29, Binding protein 53, BP 53, BP53, Growth hormone dependent binding protein , IBP 3, IBP-3, IBP3, IBP3_HUMAN, IGF binding protein 3, IGF-binding protein 3, IGFBP 3, IGFBP3, Insulin Like Growth Factor Binding Protein 3, Insulin-like growth factor binding protein 3 precursor, Insulin-like growth factor-binding protein 3

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.