Human HGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human HGF in the samples. A monoclonal antibody specific for human HGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for HGF, and HGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of HGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

1.99 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Wiederherstellung

95% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human HGF.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A; scatter factor), Hepatocyte growth factor beta chain, Hepatocyte growth factor precursor, Hepatopoietin A, Hepatopoietin-A, HGFB, HGF_HUMAN, HPTA, Lung fibroblast derived mitogen , OTTHUMP00000161349, OTTHUMP00000206710, OTTHUMP00000206711, OTTHUMP00000206712, OTTHUMP00000206713, OTTHUMP00000206730, Scatter factor, SF

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.