Human Granzyme A ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Granzyme A in the samples. A monoclonal antibody specific for human Granzyme A has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Granzyme A, and Granzyme A present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Granzyme A bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.31 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

112% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human Granzyme A.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

CTL tryptase, CTLA3, Cytolytic t cell and natural killer cell specific trypsin like serine protease, Cytotoxic T lymphocyte associated serine esterase 3, Cytotoxic T lymphocyte proteinase 1, Cytotoxic T-lymphocyte proteinase 1, Fragmentin 1, Fragmentin-1, GRAA_HUMAN, Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3, Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease), Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3), Granzyme A precursor, Granzyme-1, GZMA, H factor, Hanukah factor serine protease, Hanukkah factor, HF, HFSP, TSP1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.