Human Fas Ligand ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Fas Ligand in the samples. A monoclonal antibody specific for human Fas Ligand has been immobilized onto microwells. Standard or samples are pipetted into the wells, followd by the addition of biotin-linked monoclonal antibody specific for Fas Ligand, and Fas Ligand present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Fas Ligand bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

3.42 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

97% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human Fas Ligand.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

ALPS1B, Apoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, Apoptosis antigen ligand 1, APT1LG1, APTL, CD178, CD178 antigen, CD95 L, CD95 ligand, CD95-L, CD95L, CD95L protein, Fas antigen ligand, Fas L, Fas ligand (TNF superfamily member 6), FASL, Fasl Fas ligand (TNF superfamily member 6), FASLG, Generalized lymphoproliferative disease, Gld, soluble form, TNF superfamily member 6, TNFL6_HUMAN, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.