Human FAK Kit Elisa (A8093)

Human FAK ELISA Kit

Human FAK ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human FAK in tissue homogenates or other biological fluids.

Sandwich (quantitative)

Human FAK ELISA Kit (A8093) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human FAK in tissue homogenates or other biological fluids. An antibody specific for FAK has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the FAK present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-FAK Antibody, which binds the captured FAK present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of FAK captured in each well. The concentration of FAK can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

0.53 ng/ml

1.56-100 ng/ml

4h 30m

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

FADK, FADK 1, FAK related non kinase polypeptide, FAK1, FAK1_HUMAN, Focal adhesion Kinase, Focal adhesion kinase 1, Focal adhesion kinase isoform FAK Del33, Focal adhesion kinase related nonkinase, FRNK, p125FAK, pp125FAK, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein tyrosine kinase 2, Protein-tyrosine kinase 2, Ptk2, PTK2 protein tyrosine kinase 2