This ErbB 2 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-ErbB 2 Antibody. Standards or samples are then added to the microtire plate wells and ErbB 2, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of ErbB 2 present in the sample, Anti-ErbB 2 Antibody (Biotin) is added to each well to "sandwich" the ErbB 2 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ErbB 2 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivität

< 10 pg/ml

Detektionsbereich

62.5-4000 pg/ml

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ERBB2, ERBB2_HUMAN, HER 2, HER 2/NEU, HER2, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.