Human Dkk-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Dkk-1 in the samples. A monoclonal antibody specific for human Dkk-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Dkk-1, and Dkk-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Dkk-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

2.97 pg/ml

Detektionsbereich

62.50 - 4000 pg/ml

Wiederherstellung

100% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human Dkk-1.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1 , Dickkopf like protein 1, Dickkopf related protein 1, Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-1, Dickkopf-related protein 1, DKK 1, DKK1, DKK1_HUMAN, hDkk 1 , hDkk-1, hDkk1 , mDkk1, SK

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.