Human CX3CL1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CX3CL1 in the samples. A monoclonal antibody specific for human CX3CL1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CX3CL1, and CX3CL1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CX3CL1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

34.45 pg/ml

Detektionsbereich

156.25 - 10000 pg/ml

Wiederherstellung

88% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human CX3CL1.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

A 152E5.2, AB030188, ABCD 3, ABCD3, AI848747, C X3 C motif chemokine 1, C-X3-C motif chemokine 1, C3Xkine, Chemokine (C X3 C motif) ligand 1, Chemokine (C-X3-C motif) ligand 1, Chemokine C X3 C motif ligand 1, Chemokine CX3C Motif Ligand 1, CX3C membrane anchored chemokine, CX3C membrane-anchored chemokine, CXC 3, CXC3, CXC3C, D8Bwg0439e, FKN, Fractalkine, Neurotactin, NTN, NTT, Processed fractalkine, SCYD 1 , SCYD1, Small inducible cytokine D1, Small inducible cytokine subfamily D (Cys X3 Cys) member 1, Small inducible cytokine subfamily D (Cys X3 Cys), member 1, Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) , Small inducible cytokine subfamily D member 1, Small-inducible cytokine D1, X3CL1_HUMAN

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.