Human CD258 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CD258 in the samples. A monoclonal antibody specific for human CD258 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CD258, and CD258 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CD258 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

22.26 pg/ml

Detektionsbereich

312.50 - 20000 pg/ml

Wiederherstellung

91% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human CD258.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

CD 258, CD258 antigen, Delta transmembrane LIGHT, Herpes virus entry mediator ligand, Herpesvirus entry mediator A, Herpesvirus entry mediator ligand, herpesvirus entry mediator-ligand, HVEM L, HVEM-L, HVEML, Ligand for herpesvirus entry mediator, LIGHT, LTg, soluble form, TNF14, TNF14_HUMAN, TNFSF 14, Tnfsf14, TNFSF14 protein, TR 2, TR2, Tumor necrosis factor (ligand) superfamily, member 14, Tumor necrosis factor ligand superfamily member 14, Tumor necrosis factor ligand superfamily, member 14, Tumor necrosis factor receptor like 2, tumor necrosis factor receptor-like 2, Tumor necrosis factor superfamily member 14, Tumor necrosis factor superfamily member LIGHT

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.