Human CD117 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CD117 in the samples. A monoclonal antibody specific for human CD117 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CD117, and CD117 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CD117 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

2.16 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Spezifität

This kit can assay both natural and recombinant human CD117.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C Kit, c-Kit, c-Kit Ligand, Kit, Kit Ligand, KIT oncogene, KIT proto oncogene receptor tyrosine kinase, KIT_HUMAN, Mast cell growth factor receptor, Mast/stem cell growth factor receptor Kit, Mast/stem cell growth factor receptor precursor, MGF, p145 c-kit, PBT, Piebald trait protein, Proto oncogene c Kit, Proto oncogene tyrosine protein kinase Kit, Proto-oncogene c-Kit, SCF Receptor, SCFR, soluble KIT variant 1, Steel Factor Receptor, Stem cell factor receptor, tyrosine protein kinase Kit, Tyrosine-protein kinase Kit, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.