Human BMP-7 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-7 in the samples. A monoclonal antibody specific for human BMP-7 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BMP-7, and BMP-7 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BMP-7 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Cell culture supernatant, serum and plasma.
Sensitivität
2.06 pg/ml
Detektionsbereich
7.81 - 500 pg/ml
Wiederherstellung
98% (Average Recovery from Serum Samples).
Spezifität
This kit can assay both natural and recombinant human BMP-7.
Reaktivität
Human
Kreuzreaktivität
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2 - 8°C.
Synonyms
BMP 7, BMP7, BMP7_HUMAN, Bone morphogenetic protein 7, Bone morphogenic protein 7, Eptotermin alfa, OP 1, OP-1, OP1, Osteogenic protein 1
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
