Human BMP-6 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-6 in the samples. A monoclonal antibody specific for human BMP-6 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and BMP-6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for BMP-6 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BMP-6 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

2.33 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Wiederherstellung

90% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human BMP-6.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

BMP6, BMP6_HUMAN, Bone morphogenetic protein 6, Bone Morphogenic Protein 6, Decapentaplegic vegetal related, DVR6, HGNC:12686, TGFB related vegetal related growth factor, Transforming growth factor beta, Vegetal related (TGFB related) cytokine, Vegetal related growth factor (TGFB related), Vg related sequence, VG-1-R, VG-1-related protein, Vg1 related sequence, VGR, VGR-1, VGR1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.