Human Apolipoprotein B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo B in the samples. A monoclonal antibody specific for human Apo B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo B, and Apo B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Cell culture supernatant, serum and plasma.
Sensitivität
74. pg/ml
Detektionsbereich
312.50 - 20000 pg/ml
Spezifität
This kit can assay both natural and recombinant human Apo B.
Reaktivität
Human
Kreuzreaktivität
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2 - 8°C.
Synonyms
Apo B-100, Apo B-48, APOB, ApoB 100 , ApoB 48, APOB_HUMAN, Apolipoprotein B (including Ag(x) antigen), Apolipoprotein B 100, Apolipoprotein B-48, Apolipoprotein B48, FLDB, LDLCQ4
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
