Human Adiponectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Adiponectin in the samples. A monoclonal antibody specific for human Adiponectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Adiponectin, and Adiponectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Adiponectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.44 pg/ml

Detektionsbereich

62.50 - 4000 pg/ml

Wiederherstellung

99% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human Adiponectin.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, adipocyte-specific secretory protein, Adiponectin precursor, adiponectin, C1Q and collagen domain containing, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPO_HUMAN, ADIPQTL1, ADPN, APM 1, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein , Gelatin binding protein 28, Gelatin-binding protein

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.