Anti-Hsp20 Antibody [ARC1787] (A305617)

Name: Anti-Hsp20 Antibody [ARC1787]
Brand: Antibodies.com
SKU: A305617
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1787] antibody to Hsp20 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Hsp20 Antibody [ARC1787]

Description

Rabbit monoclonal [ARC1787] antibody to Hsp20.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human HSP20/HSPB6 (O14558).

Sequence

DPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPAS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1787

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Hsp20 Antibody [ARC1787] (A305617)

Western blot analysis of extracts of various cell lines, using Anti-Hsp20 Antibody [ARC1787] (A305617) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Hsp20 Antibody [ARC1787] (A305617)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Hsp20 Antibody [ARC1787] (A305617) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Hsp20 Antibody [ARC1787] (A305617)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Hsp20 Antibody [ARC1787] (A305617) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Hsp20 Antibody [ARC1787] (A305617)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Hsp20 Antibody [ARC1787] (A305617) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.