Rabbit polyclonal antibody to HSF1 (phospho-Ser303)
Spezifität
The antibody detects endogenous level of HSF1 only when phosphorylated at serine 303.
Anwendungen
WB, IHC
Verdünnungen
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reaktivität
Human
Immunogen
Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1.
Wirt
Rabbit
Klonalität
Polyclonal
Konjugat
Unconjugated
Reinigung
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Konzentration
1.0mg / ml
Geschätztes MW
82kd
Formulierung
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Lagerung
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from MCF7 cells using HSF1 (phospho-Ser303) Antibody #11263 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Phospho-Ser303) Antibody #11263 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody #11263.
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