Unconjugated
Four hybridomas making monoclonal antibodies against horseradish peroxidase have been established. Two of them are IgM and two are IgG1 with kappa light chains. Antibody HP-03 has been selected as the most suitable one for preparation of the peroxidase-anti-peroxidase complex. Furthermore, optimal conditions for construction and applications of the PAP complex in immunocytochemical and immunohistochemical methods are shown.