This hnRNPM Cell Based ELISA Kit allows for the detection of hnRNPM and the effects that certain stimulation conditions have on hnRNPM expression in different cell lines. Qualitative determination of hnRNPM concentration is achieved by an indirect ELISA format. In essence, the hnRNPM is captured by Anti-hnRNPM Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this hnRNPM Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
CEA receptor, CEAR, Heterogeneous nuclear ribonucleoprotein M, hnRNP M, HNRNPM4, HNRPM, HNRPM4, HNRPM_HUMAN, HTGR1, N-acetylglucosamine receptor 1, NAGR1
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 4: Western Blot - hnRNPM Cell Based ELISA Kit (CB5911)
The mouse monoclonal antibody to GAPDH is used as a positive control inhnRNPM Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
