Anti-HMGB1 Antikörper [ARC0001] (A308594)

Name: Anti-HMGB1 Antibody [ARC0001]
Brand: Antibodies.com
SKU: A308594
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC0001] Kaninchen Antikörper gegen HMGB1 für WB, IHC und ICC/IF.

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Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Knockout-validierter Antikörper

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Produktdetails

Produktname

Anti-HMGB1 Antibody [ARC0001]

Beschreibung

Rabbit monoclonal [ARC0001] antibody to HMGB1.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429).

Sequenz

SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC0001

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

25 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1_HUMAN, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

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Figure 1: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts of various cell lines, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts from wild type (WT) and HMGB1 knockout (KO) 293T cells, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

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Figure 4: Immunohistochemistry - Anti-HMGB1 Antibody [ARC0001] (A308594)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-HMGB1 Antibody [ARC0001] (A308594) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.