This antibody recognizes a common extracellular epitope on human HLA DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, and macrophages).

Anwendungen

Flow Cytometry

Verdünnungen

Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reaktivität

Human

Immunogen

Thymocyte membrane.

Wirt

Mouse

Klonalität

Monoclonal

Klon

MEM-12

Isotyp

IgG1

Konjugat

Excitation: 565nm, Emission: 578nm

Reinigung

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyme

HLA DP, HLA DR + HLA DP, MHC Class II

Isotyp-Kontrollantikörper

Mouse IgG1 [MOPC-21] (PE) (A86199)

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

Bild Vergrößern

Figure 1: Flow Cytometry - Anti-HLA DR Antibody [MEM-12] (PE) (A85811)

Surface staining of human peripheral blood cells with Anti-HLA DR Antibody (A85811).

Produktreferenzen für diesen Klon (1)

Publikation anzeigen

Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.

Verweise: Anti-HLA DR Antibody [MEM-12] (PE) (ab28327)

Abstrakt:

Seven murine monoclonal antibodies were prepared that react with human class II antigens. Four of them (HL-39, MEM-12, MEM-24G, and MEM-32B: react with a monomorphic determinant dependent on association of heavy and light chains of DR antigens, two others (HL-38 and HL-40) recognize a monomorphic determinant localized on the light chains of DR and DP antigens. The antibody HL-37 is directed against a determinant present on DQ1 and DQ3, but not DQ2 molecules; at least in the case of DQ1, the epitope recognized is located on the light chain.

Publikation anzeigen