Anti-Histone H3.3 Antikörper [ARC0255] (A306483)

Name: Anti-Histone H3.3 Antibody [ARC0255]
Brand: Antibodies.com
SKU: A306483
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC0255] Kaninchen Antikörper gegen Histone H3.3 für WB, IHC, IP, ChIP und ChIP-seq.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

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Produktdetails

Produktname

Anti-Histone H3.3 Antibody [ARC0255]

Beschreibung

Rabbit monoclonal [ARC0255] antibody to Histone H3.3.

Anwendungen

WB, IHC, IP, ChIP, ChIP-seq

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, ChIP-seq: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 57-136 of human Histone H3.3 (P84243).

Sequenz

KSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC0255

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

17 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B (H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

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Figure 1: Chromatin Immunoprecipitation - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Chromatin immunoprecipitations analysis of cross-linked chromatin from HeLa cells, using Anti-Histone H3.3 Antibody [ARC0255] (A306483). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.

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Figure 2: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of Rat lung, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.