Mouse monoclonal [HRB2/258] antibody to HER2 (CF® 488).
Specificity
This antibody recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reactivity
Human, Rat
Immunogen
Recombinant extracellular domain of human HER-2 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
HRB2/258
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF® 488
Excitation: 490nm, Emission: 515nm
Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
185 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
