Anti-Heme Oxygenase 1 Antikörper [ARC53508] (A307278)

Name: Anti-Heme Oxygenase 1 Antibody [ARC53508]
Brand: Antibodies.com
SKU: A307278
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC53508] Kaninchen Antikörper gegen Heme Oxygenase 1 für WB, IHC und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper ER Marker

Produktdetails

Produktname

Anti-Heme Oxygenase 1 Antibody [ARC53508]

Beschreibung

Rabbit monoclonal [ARC53508] antibody to Heme Oxygenase 1.

Anwendungen

WB, IHC, IP

Verdünnungen

WB: 1:2,000-1:10,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1).

Sequenz

MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQA

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC53508

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

33 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

32 kD, bK286B10, D8Wsu38e, Heat shock protein, heat shock protein 32 kD, heat shock protein 32kD, Heme oxygenase (decycling) 1, Hemox, Hmox, HMOX 1, Hmox1, HMOX1_HUMAN, HO, HO 1, HO-1, HO1, Hsp32

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (10)

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Figure 1: Western Blot - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Western blot analysis of extracts of various cell lines, using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Western blot analysis of extracts of Raw264. 7 cells, using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Western Blot - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Western blot analysis of extracts of HepG2 cells, using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Western Blot - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Western blot analysis of extracts from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockout (KO) HeLa cells, using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 5: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 9: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 10: Western Blot - Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278). This Western blot was performed on the immunoprecipitate using Anti-Heme Oxygenase 1 Antibody [ARC53508] (A307278) at a dilution of 1:1000.

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