Anti-HDAC7 Antibody [PCRP-HDAC7-1B6] - BSA and Azide free
Beschreibung
Mouse monoclonal [PCRP-HDAC7-1B6] antibody to HDAC7.
Spezifität
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.
Anwendungen
Flow Cytometry
Positivkontrolle
HeLa cells.
Verdünnungen
Flow Cytometry: 1-2 µg/million cells
Reaktivität
Human
Immunogen
Recombinant full-length human HDAC7 protein.
Wirt
Mouse
Klonalität
Monoclonal
Klon
PCRP-HDAC7-1B6
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
1 mg/ml
Molekulargewicht
105 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.