Figure 1: Western Blot - Anti-HDAC6 Antibody [ARC0805] (A307941)
Western blot analysis of extracts of various cell lines, using Anti-HDAC6 Antibody [ARC0805] (A307941) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-HDAC6 Antibody [ARC0805] (A307941) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-HDAC6 Antibody [ARC0805] (A307941)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-HDAC6 Antibody [ARC0805] (A307941). This Western blot was performed on the immunoprecipitate using Anti-HDAC6 Antibody [ARC0805] (A307941) at a dilution of 1:1000.
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