ChIP of 2µg Anti-Hdac1 Antibody (A82481) with MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
Figure 2: Western Blot - Anti-HDAC1 Antibody (A82481)
Anti-Hdac1 Antibody (A82481) (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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