Anti-GSK3 beta (phospho Ser9) Antikörper [ARC0069] (A308989)

Western blot analysis of extracts of various cell lines, using Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.