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Anti-GRP78 Antikörper (A305100)

Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com
$520
Product Datasheet

Polyklonal Kaninchen Antikörper gegen GRP78 für WB, IHC, ICC/IF und IP.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

Telefon
+1 (314) 370-6046
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Email
orders@antibodies.com

Produktname
Anti-GRP78 Antibody
Beschreibung
Rabbit polyclonal antibody to GRP78.
Anwendungen
WB, IHC, ICC/IF, IP
Verdünnungen
WB: 1:1,000, ICC/IF: 1:100
Reaktivität
Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus, Canine
Immunogen
Rat GRP78 (Bip) synthetic peptide conjugated to KLH.
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Peptide affinity purification.
Konzentration
1 mg/ml
Molekulargewicht
~78 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78 BiP, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-GRP78 Antibody (A305100)
Western blot analysis of rat tissue lysates showing detection of GRP78 protein using Anti-GRP78 Antibody (A305100) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.comBild Vergrößern
Figure 2: Immunofluorescence - Anti-GRP78 Antibody (A305100)
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.comBild Vergrößern
Figure 3: Immunofluorescence - Anti-GRP78 Antibody (A305100)
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.comBild Vergrößern
Figure 4: Western Blot - Anti-GRP78 Antibody (A305100)
Western blot analysis of Human, Dog, mouse Cell line lysates showing detection of GRP78 protein using Anti-GRP78 Antibody (A305100) at 1:1,000.

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