Anti-GRP78 Antibody (A305023)

Name: Anti-GRP78 Antibody
Brand: Antibodies.com
SKU: A305023
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to GRP78 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-GRP78 Antibody

Description

Rabbit polyclonal antibody to GRP78.

Specificity

This antibody has weak detection in human tissues.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:60

Reactivity

Human, Mouse, Rat

Immunogen

His-tagged rat GRP78.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1 mg/ml

Molecular Weight

~78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Endoplasmic reticulum chaperone BiP, GRP-78, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, HSP70 family protein 5, HSPA5, Immunoglobulin heavy chain-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-GRP78 Antibody (A305023)

Western blot analysis of rat brain cell lysates showing detection of ~78 kDa GRP78 protein using Anti-GRP78 Antibody (A305023) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~78 kDa.

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Immunofluorescence - Anti-GRP78 Antibody (A305023)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305023), at 1:60 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Immunofluorescence - Anti-GRP78 Antibody (A305023)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305023), at 1:60 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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