This GRK5 Cell Based ELISA Kit allows for the detection of GRK5 and the effects that certain stimulation conditions have on GRK5 expression in different cell lines. Qualitative determination of GRK5 concentration is achieved by an indirect ELISA format. In essence, the GRK5 is captured by Anti-GRK5 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this GRK5 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
FLJ39780, FP2025, G protein coupled receptor kinase 5, G protein coupled receptor kinase GRK5, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GPRK5, GRK-pan, GRK5_HUMAN, Pan-GRK
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Western Blot - GRK5 Cell Based ELISA Kit (CB5877)
Western blot analysis of extracts from HeLa/HepG2/HuvEc cells, using Anti-GRK5 Antibody. The lane on the right is treated with the synthesized peptide.
Figure 4: Western Blot - GRK5 Cell Based ELISA Kit (CB5877)
The mouse monoclonal antibody to GAPDH is used as a positive control inGRK5 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
