Mouse monoclonal [8E9] antibody to Glucocorticoid Receptor.
Spezifität
This antibody recognises the human glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1. Human GR is a 777 amino acid ~97kDa member of the NR3 subfamily of nuclear hormone receptors, bearing a single nuclear receptor DNA-binding domain. Multiple isoforms of the human glucocorticoid receptor are generated by either alternative splicing or alternative initiation (UniProt:: P04150).In the absence of bound ligand GRs are located in the cytoplasm and are translocated to the nucleus or mitochondrion following ligation. GRs are associated with heat shock proteins in the cytoplasm when ligated to steroid hormone, being disrupted on translocation of the steroid:receptor complex to the nucleus. recognises human GR, binding to an epitope between amino acids 167-176 and is therefore expected to bind all described isoforms of the human glucocorticoid receptor. Mouse anti Human glucocorticoid receptor antibody, clone 8E9 has been used successfully for the identification of human glucocorticoid receptor using flow cytometry , western blotting and immunoprecipitation where it has also been shown to bind to the murine GR (Paul-Clark et al. 2003, Bartis et al. 2007).
Anwendungen
WB, ELISA
Verdünnungen
ELISA: 1:1,000, WB: 1:1,000
Reaktivität
Human, Mouse
Immunogen
A peptide corresponding to amino acids 150-176 of human GCR linked to human thyroglobulin.
Sequenz
APTEKEFPKTHSDVSSEQQHLKGQTGT
Wirt
Mouse
Klonalität
Monoclonal
Klon
8E9
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Protein G affinity chromatography of tissue culture supernatant.
Konzentration
1 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Lagerung
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Allgemeine Hinweise
Mouse anti Human glucocorticoid receptor antibody, clone 8E9 recognizes the human glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1. Human GR is a 777 amino acid ~97kDa (Moraes et al. 2005) member of the NR3 subfamily of nuclear hormone receptors, bearing a single nuclear receptor DNA-binding domain. Multiple isoforms of the human glucocorticoid receptor are generated by either alternative splicing or alternative initiation (UniProt:: P04150).In the absence of bound ligand GRs are located in the cytoplasm and are translocated to the nucleus or mitochondrion following ligation (Rossini et al. 1984). GRs are associated with heat shock proteins in the cytoplasm when ligated to steroid hormone, being disrupted on translocation of the steroid:receptor complex to the nucleus (Tse et al. 2011). Mouse anti Human glucocorticoid receptor antibody, clone 8E9 was raised against a conserved region of the glucocorticoid receptor and recognizes human GR, binding to an epitope between amino acids 167-176 and is therefore expected to bind all described isoforms of the human glucocorticoid receptor.Mouse anti Human glucocorticoid receptor antibody, clone 8E9 has been used successfully for the identification of human glucocorticoid receptor using flow cytometry (Berki et al. 1998), western blotting (Moraes et al. 2005) and immunoprecipitation where it has also been shown to bind to the murine GR (Paul-Clark et al. 2003, Bartis et al. 2007).
Synonyme
GCCR, GCR, GCRST, GCR_HUMAN, glucocorticoid nuclear receptor variant 1, GR, GRL, Grl1, nr3c1, Nuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)