Anti-Gli3 Antibody [ARC1941] (A308202)

Name: Anti-Gli3 Antibody [ARC1941]
Brand: Antibodies.com
SKU: A308202
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1941] antibody to Gli3 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Gli3 Antibody [ARC1941]

Description

Rabbit monoclonal [ARC1941] antibody to Gli3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 (P10071).

Sequence

PNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1941

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

190 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, Transcriptional activator GLI3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Gli3 Antibody [ARC1941] (A308202)

Western blot analysis of extracts of HeLa cells, using Anti-Gli3 Antibody [ARC1941] (A308202) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-Gli3 Antibody [ARC1941] (A308202)

Immunofluorescence analysis of C6 cells using Anti-Gli3 Antibody [ARC1941] (A308202) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Gli3 Antibody [ARC1941] (A308202)

Immunofluorescence analysis of U-2 OS cells using Anti-Gli3 Antibody [ARC1941] (A308202) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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