Anti-GIT1 Antikörper [S39B-8] (A304764)

Name: Anti-GIT1 Antibody [S39B-8]
Brand: Antibodies.com
SKU: A304764
Price: 500.0 USD
Availability: InStock

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$500

Monoklonal [S39B-8] Maus Antikörper gegen GIT1 für WB, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-GIT1 Antibody [S39B-8]

Beschreibung

Mouse monoclonal [S39B-8] antibody to GIT1.

Anwendungen

WB, ICC/IF, IP

Verdünnungen

WB: 1:1,000

Reaktivität

Human, Mouse, Rat

Kreuzreaktivität

This antibody does not cross-react with GIT2.

Immunogen

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1.

Wirt

Mouse

Klonalität

Monoclonal

Klon

S39B-8

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~90 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ARF GAP GIT1, ARF GTPase activating protein GIT1, ARF GTPase-activating protein GIT1, CAT 1, CAT-1, CaT1, Cool associated and tyrosine phosphorylated protein 1, Cool-associated and tyrosine-phosphorylated protein 1, G protein coupled receptor kinase interacting ArfGAP 1, G protein coupled receptor kinase interactor 1, G protein-coupled receptor kinase-interactor 1, GIT1_HUMAN, GRK interacting protein 1, GRK-interacting protein 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-GIT1 Antibody [S39B-8] (A304764)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GIT1 Antibody [S39B-8] (A304764), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-GIT1 Antibody [S39B-8] (A304764)

Western blot analysis of rat brain membrane lysate showing detection of GIT1 protein using Anti-GIT1 Antibody [S39B-8] (A304764) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-GIT1 Antibody [S39B-8] (A304764)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GIT1 Antibody [S39B-8] (A304764), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.