Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ARF GAP GIT1, ARF GTPase-activating protein GIT1, CAT-1, CAT1, Cool-associated and tyrosine-phosphorylated protein 1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, p95-APP1
Western blot analysis of extracts of various cell lines, using Anti-GIT1 Antibody (A92785) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using Anti-GIT1 Antibody (A92785) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.