This antibody reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Anwendungen
IHC-P, IP, WB, ICC
Verdünnungen
IHC-P: 10 µg/ml; Positive Control: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues, IHC: 5-10 µg/ml.
Reaktivität
Porcine, Feline, Human
Kreuzreaktivität
This antibody does not cross react with Rat.
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Wirt
Mouse
Klonalität
Monoclonal
Klon
GF-01
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Protein A chromatography.
Konzentration
1 mg/ml
Geschätztes MW
55 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.