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Anti-GFAP Antikörper [ARC0206] (A307282)

Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Immunofluorescence - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com Immunofluorescence - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com +2
$470
Product Datasheet

Monoklonal [ARC0206] Kaninchen Antikörper gegen GFAP für WB, IHC und ICC/IF.

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Versandkosten
$40
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Lieferung in 5-8 Werktagen

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Produktname
Anti-GFAP Antibody [ARC0206]
Beschreibung
Rabbit monoclonal [ARC0206] antibody to GFAP.
Anwendungen
WB, IHC, ICC/IF
Verdünnungen
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:500-1:1,000
Reaktivität
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GFAP (P14136).
Sequenz
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAE
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC0206
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
48 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, gfapl, GFAP_HUMAN, Glial fibrillary acidic protein, Intermediate filament protein, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-GFAP Antibody [ARC0206] (A307282)
Western blot analysis of extracts of various cell lines, using Anti-GFAP Antibody [ARC0206] (A307282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-GFAP Antibody [ARC0206] (A307282)
Western blot analysis of extracts of U-251MG cells, using Anti-GFAP Antibody [ARC0206] (A307282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comBild Vergrößern
Figure 3: Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-GFAP Antibody [ARC0206] (A307282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comBild Vergrößern
Figure 4: Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-GFAP Antibody [ARC0206] (A307282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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