Anti-Geminin Antikörper [GMNN/4033] (A278323)

Anti-Geminin Antibody [GMNN/4033] - BSA and Azide free

Mouse monoclonal [GMNN/4033] antibody to Geminin.

Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

IHC-P

IHC-P: 1-2 µg/ml

Human

Recombinant fragment, around amino acids 71-202, of human Geminin protein. The exact sequence is proprietary.

Mouse

Monoclonal

GMNN/4033

IgG1

Unconjugated

Protein A/G chromatography.

1 mg/ml

35 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Geminin Antibody [GMNN/4033] (A277735).

DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GEMI_HUMAN, GMNN, OTTHUMP00000039393, RP3 369A17.3