Anti-Geminin Antikörper [GMNN/3665] (A278322)

Name: Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free
Brand: Antibodies.com
SKU: A278322
Price: 525.0 USD
Availability: InStock

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$525

Monoklonal [GMNN/3665] Maus Antikörper gegen Geminin für IF und IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Monospezifischer Antikörper

Produktdetails

Produktname

Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free

Beschreibung

Mouse monoclonal [GMNN/3665] antibody to Geminin.

Spezifität

Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Anwendungen

IF, IHC-P

Verdünnungen

IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reaktivität

Human

Immunogen

Recombinant fragment corresponding to human Geminin protein. The exact sequence is proprietary.

Wirt

Mouse

Klonalität

Monoclonal

Klon

GMNN/3665

Isotyp

IgG1

Leichte Ketten

kappa

Konjugat

Unconjugated

Reinigung

Protein A/G chromatography.

Konzentration

1 mg/ml

Molekulargewicht

35 kDa

Produktform

Liquid

Formulierung

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Allgemeine Hinweise

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Geminin Antibody [GMNN/3665] (A277734).

Synonyme

DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GEMI_HUMAN, GMNN, OTTHUMP00000039393, RP3 369A17.3

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Immunohistochemistry - Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free (A278322)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue using Anti-Geminin Antibody [GMNN/3665]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.

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Figure 2: Immunohistochemistry - Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free (A278322)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue using Anti-Geminin Antibody [GMNN/3665].

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Figure 3: Immunofluorescence - Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free (A278322)

Immunofluorescent analysis of PFA-fixed MCF-7 cells stained with Anti-Geminin Antibody [GMNN/3665] followed by Goat Anti-Mouse IgG (CF® 488) (Green).

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Figure 4: Anti-Geminin Antibody [GMNN/3665] - BSA and Azide free (A278322)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Geminin Antibody [GMNN/3665]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.