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Anti-GATA3 Antikörper [ARC0115] (A307313)

Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com
$470
Product Datasheet

Monoklonal [ARC0115] Kaninchen Antikörper gegen GATA3 für WB, IP und ChIP.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com

Produktname
Anti-GATA3 Antibody [ARC0115]
Beschreibung
Rabbit monoclonal [ARC0115] antibody to GATA3.
Anwendungen
WB, IP, ChIP
Verdünnungen
WB: 1:500-1:2,000, IP: 1:100-1:500, ChIP: 1:100-1:500
Reaktivität
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Sequenz
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC0115
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
48 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
GATA 3, GATA binding factor 3, GATA binding protein 3, GATA-binding factor 3, GATA3_HUMAN, HDR, HDRS, MGC2346, MGC5199, MGC5445, Trans acting T cell specific transcription factor GATA 3, Trans-acting T-cell-specific transcription factor GATA-3
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Western blot analysis of extracts of various cell lines, using Anti-GATA3 Antibody [ARC0115] (A307313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATA3 Antibody [ARC0115] (A307313). This Western blot was performed on the immunoprecipitate using Anti-GATA3 Antibody [ARC0115] (A307313) at a dilution of 1:500.
Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comBild Vergrößern
Figure 3: Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313)
Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 cells, using Anti-GATA3 Antibody [ARC0115] (A307313) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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