Anti-GAPDH Antikörper [ARC50888] (A309068)

Anti-GAPDH Antikörper [1D4]

A85382

229 Produktreferenzen

Monoklonal [1D4] Maus Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Anti-GAPDH Antikörper [GA1R]

A85271

18 Produktreferenzen

Monoklonal [GA1R] Maus Antikörper gegen GAPDH für Dot, ELISA, IS und WB.

(4)

A85377

Polyklonal Kaninchen Antikörper gegen GAPDH für WB, ICC/IF und IHC.

A83722

Polyklonal Ziege Antikörper gegen GAPDH für ELISA, WB, IF und IHC.

Anti-GAPDH Antikörper [1D4]

A85382

229 Produktreferenzen

Monoklonal [1D4] Maus Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Anti-GAPDH Antikörper [GA1R]

A85271

18 Produktreferenzen

Monoklonal [GA1R] Maus Antikörper gegen GAPDH für Dot, ELISA, IS und WB.

(4)

A85377

Polyklonal Kaninchen Antikörper gegen GAPDH für WB, ICC/IF und IHC.

A83722

Polyklonal Ziege Antikörper gegen GAPDH für ELISA, WB, IF und IHC.

Produktdetails

Produktname

Anti-GAPDH Antibody [ARC50888]

Beschreibung

Rabbit monoclonal [ARC50888] antibody to GAPDH.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:10,000-1:1:120,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).

Sequenz

VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC50888

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

36 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (12)

Figure 1: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of extracts of HeLa cells, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 2: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of various lysates, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 3: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 4: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 5: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 6: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 7: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 8: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 9: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 10: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of NIH/3T3 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Figure 11: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of PC-12 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).