Anti-GAD67 Antikörper (A82616)

Name: Anti-GAD67 Antibody
Brand: Antibodies.com
SKU: A82616
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Novus (NBP1-06989). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen GAD67 für ELISA, WB, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-GAD67 Antibody

Beschreibung

Goat polyclonal antibody to GAD67.

Anwendungen

ELISA, WB, IF, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 0.3-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human, Mouse, Rat

Kreuzreaktivität

This antibody is not expected to cross react with GAD2.

Immunogen

Synthetic peptide corresponding to the internal region of human GAD67.

Sequenz

C-PDSPQRREKLHK

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

70 kDa

Geschätztes MW

66.9kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, DCE1_HUMAN, EC 4.1.1.15, FLJ45882, GAD, GAD 67, GAD-67, GAD1, Glutamate decarboxylase 1, Glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 1 brain 67kD, Glutamate decarboxylase 1 brain 67kDa, Glutamate decarboxylase 67 kDa isoform, Glutamate decarboxylase, brain, 67-KD, OTTHUMP00000041055, SCP

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-GAD67 Antibody (A82616)

GAD67 expression in Human Cerebellum (A), Mouse Brain (B), and Rat Brain (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-GAD67 Antibody (A82616) at 1µg/ml (A-B) or 0.3µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-GAD67 Antibody (A82616)

GAD67 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GAD67 Antibody (A82616) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-GAD67 Antibody (A82616)

GAD67 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-GAD67 Antibody (A82616) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.