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Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)

Validation Data - Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)
$770
Product Datasheet

Mouse monoclonal [FN12-8] antibody to Fibronectin for WB, ELISA, Inhibition Assay and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Fibronectin Antibody [FN12-8] - Azide free
Description
Mouse monoclonal [FN12-8] antibody to Fibronectin.
Specificity
Recognizes an epitope in the cell-binding domain. Can inhibit cell adhesion.
Applications
WB, ELISA, Inhibition Assay, IHC
Dilutions
WB: 1-10 µg/ml, IHC-P: 5-10 µg/ml, IHC-Fr: 5-10 µg/ml, ELISA: 1-10 µg/ml, Cell Adhesion Inhibition Assay: 0.1-1 µg/ml
Reactivity
Human, Bovine
Immunogen
Human plasma Fibronectin.
Host
Mouse
Clonality
Monoclonal
Clone ID
FN12-8
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 200µl of double distilled water (2mg/ml final concentration).
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 1% BSA.
Storage
Lyophilized: Store at 4°C. Reconstituted: Aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
For IHC use proteinase K treatment for antigen retrieval.WB under reducing or non-reducing conditions.
Synonyms
CIG, Cold-insoluble globulin, FN, FN1
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Validation Data - Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)Enlarge Image

Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)

IHC detection of Fibronectin using A304547 at 1-10µg/ml on paraffin-embedded colon cancer tissue following Proteinase K treatment for antigen retrieval. Staining of Fibronectin in human well-differentiated colon cancer tissue (lower) and moderately differentiated colon cancer (upper).
Publishing research using Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)? Please let us know so that we can list the citation on this page.

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