Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free
Beschreibung
Recombinant rabbit monoclonal [DM154] antibody to Fibroblast Activation Protein alpha.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Rabbit
Klonalität
Monoclonal
Klon
DM154
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Figure 1: Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free (A318554)
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human Fibroblast Activation Protein alpha Protein (6×His Tag) (A318116) can bind Anti-Fibroblast Activation Protein alpha Antibody [DM154] - Azide free (A318554) in a linear range of 1-500 µg/ml.
Figure 2: Flow Cytometry - Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free (A318554)
Flow cytometry analysis with Anti-Fibroblast Activation Protein alpha Antibody [DM154] - Azide free (A318554) on Expi293 cells transfected with human FAP (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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