Figure 1: Western Blot - Anti-Ezrin (phospho Tyr353) Antibody (A308302)
Western blot analysis of extracts of 293T cells, using Anti-Ezrin (phospho Tyr353) Antibody (A308302) at 1:1,000 dilution or Ezrin antibody (A0703). 293T cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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