Anti-Estrogen Receptor beta Antikörper (A83108)

Name: Anti-Estrogen Receptor beta Antibody
Brand: Antibodies.com
SKU: A83108
Price: 480.0 USD
Availability: InStock

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$480

Polyklonal Ziege Antikörper gegen Estrogen Receptor beta für ELISA, WB und IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

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Produktdetails

Produktname

Anti-Estrogen Receptor beta Antibody

Beschreibung

Goat polyclonal antibody to Estrogen Receptor beta.

Anwendungen

ELISA, WB, IF

Verdünnungen

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 417-428, of human Estrogen Receptor beta.

Sequenz

C-TQDADSSRKLAH

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

50 kDa

Geschätztes MW

53.0kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

ER BETA, ER-beta, Erb, ESR B, ESR BETA, ESR2, ESR2_HUMAN, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, Estrogen receptor 2, estrogen receptor 2 (ER beta), estrogen receptor beta 4, NR3A2, Nuclear receptor subfamily 3 group A member 2

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in Human Placenta lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.