Figure 1: Western Blot - Anti-Eph receptor A2 (phospho Tyr594) Antibody (A91407)
Western blot analysis of extracts of SH-SY5Y cells, using Anti-Eph receptor A2 (phospho Tyr594) Antibody (A91407) at 1:1,000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/ml) for 5 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
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