Figure 1: Western Blot - Anti-EPF Antibody (A305094)
Western blot analysis of rat brain cell lysates showing detection of Cpn10 protein using Anti-EPF Antibody (A305094) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-EPF Antibody (A305094) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..
