This antibody recognizes an extracellular epitope within EGF-like domain I of CD326, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Anwendungen

Flow Cytometry

Verdünnungen

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reaktivität

Human

Immunogen

Small cell lung carcinoma cell line H69.

Wirt

Mouse

Klonalität

Monoclonal

Klon

VU-1D9

Isotyp

IgG1

Konjugat

PerCP-Cyanine 5.5

Excitation: 482nm, Emission: 695nm

Reinigung

This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyme

17 1A, 323/A3, Adenocarcinoma associated antigen, Adenocarcinoma-associated antigen, Antigen identified by monoclonal, AUA1, CD326, CD326 antigen, Cell surface glycoprotein Trop 1, Cell surface glycoprotein Trop 2, Cell surface glycoprotein Trop-1, CO 17A, CO17 1A, CO17A, DIAR5, EGP, EGP 2, EGP2, EGP314, EGP40, Ep CAM, Ep-CAM, EpCAM1, EPCAM_HUMAN, Epithelial cell adhesion molecule, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, ESA, GA733 1, GA733 2, GA733-2, gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein, gp4, hEGP 2, hEGP314, HNPCC8, Human epithelial glycoprotein 2, KS 1/4 antigen, KS1/4, KSA, Ly74, Lymphocyte antigen 74, M1S 1, M1S2, M4S1, Major gastrointestinal tumor associated protein GA733 2, Major gastrointestinal tumor-associated protein GA733-2, mEGP314, Membrane component chromosome 4 surface marker (35kD glycoprotein), Membrane component, chromosome 4, surface marker, Membrane component, chromosome 4, surface marker 1, MIC18, MK 1, Protein 289A, TACD1, TACSTD1, TROP1, Tumor associated calcium signal transducer 1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1

Isotyp-Kontrollantikörper

Mouse IgG1 [MOPC-21] (PerCP-Cyanine 5.5) (A122133)

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

Bild Vergrößern

Figure 1: Flow Cytometry - Anti-EpCAM Antibody [VU-1D9] (PerCP-Cyanine 5.5) (A121947)

Flow cytometric analysis of human MCF-7 cell line with Anti-CD326 Antibody [VU-1D9] (PerCP-Cyanine 5.5).

Produktreferenzen für diesen Klon (1)

Bild Vergrößern

(2)

Optofluidic device for the quantification of circulating tumor cells in breast cancer

Verweise: Anti-EpCAM Antibody [VU-1D9] (PerCP-Cyanine 5.5) (ab157319)

Abstrakt:

Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.

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